Potential antimicrobial efficiency of propolis extract (bee glue) was experimentally studied on gram positive and negative bacterias by manufacturing propolis extract-based textiles. Pre-samples were prepared by varying percentange concentration of propolis extract in PVA polymer solution and homojenic solutions were electrospun onto polypropylene nonwoven fabric. In-vitro experiments showed that antimicrobial efficiency of extract-containing nanocamposite samples were better than those of not including. According to investigations nanocomposite fabrics with propolis extract sol. were provided antimicrobial effect against to gram positive bacteria (S. aureus) but not to gram negative bacteria (A baumannii and P. aeruginosa). The results indicated that the electrospun PVA/propolis extract nanocomposites provided a good means for healing of wounds or decreasing infection proliferation caused by gram positive bacteria.