The influence of relative humidity (RH) on quasistatic current-voltage (I-V) characteristics of Bifidobacterium animalis subsp. lactis BB-12 thin layers was studied for the first time. The value of electrical conductivity in 75% RH was found to be in the order of 10 −7 (ohm•cm) −1 , which was 10 6 orders of magnitude higher than that observed in dry atmosphere. It was concluded that RH played a key role in hysteresis behavior of the measured (I-V) characteristics. FTIR measurements showed that under water moisture environment, the associated bonds between amine and carboxyl group were greatly strengthened that was the source of free charge carries after ionization. The surface charge of Bifidobacterium animalis subsp. lactis BB-12 was found to be negative by zeta potential measurements, claiming that electrons were the charge carriers.

Single strain probiotic bifidobacteria approach is promising for the future in health and non-health fields. Recent studies show that intestinal lumen microbial content and tissue microbial content are different, so the personalized microbiome approach with the 16S rRNA analysis comes to the fore with the single strain probiotic bifidobacteria (BB-12,Infantis) approach. In addition to their immune modulation effect, they have beneficial effects such as preventing pathogens from binding to the intestinal mucosa via the biofilm layer they produce, and also their electrophysical properties in various atmospheric conditions, They have the ability to be used in non-health areas such as microplastic biodegradition, nanostructures, food and agriculture fields. The availability of single strain probiotic bifidobacteria in health, ecological and food systems are signs that progress in the single strain probiotic bacteria approach will be more accurate.