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Beta-thalassemia major (β-TM) is a severe form of thalassemia caused by mutations in the β-globin gene, resulting in partial or complete deficiency of β-globin chains. This deficiency results in oxidative stress, dyserythropoiesis, and chronic anemia. Cytokine dependent hematopoietic cell linker (CLNK) belongs to the adaptor protein family and has the capacity to interact with multiple signaling proteins thereby modulating signal transduction. The aim of the present study was to examine CLNK in sera of β-TM patients and examine its association with iron overload biomarkers. Sixty β-TM patients, aged 3–12 years old and undergoing blood transfusions, and 30 healthy control children were recruited and CLNK, ferritin and iron status parameters were measured. The results showed a significant increase (p < 0.001) in serum CLNK levels in β-TM patients as compared with normal controls. The increased levels of CLNK were significantly associated with increased ferritin levels. Increased CLNK levels in β-TM may be explained by reciprocal effects between immune signaling and immature erythrocytes, which, release soluble receptors and signaling molecules, including CLNK, in the blood.

A New Azo-base-ligand was prepared [4-(( (Z)-(2,4-diclorophenyl)diazenyl)-3-((Z)-2-oxoindoline-3-ylidene)amino), (4DCPD)]-(Pyrimidine-2-yl)benzenesulfonamide). This ligand (4DCPS) with the metal ions Co(II), Ni(II) and Cu was also prepared in reaction to three chelate complexes (II). UV-Vis. Ligand along with its metal complexes were investigated based on spectra. In comparison to the one from free ligand, the (UV-Vis) electronic complex spectra showed bathochromic shifting. The Free ligand spectrum Mass & 1HNMR has been taken and the Ligand FTIR spectra and its chelating complexes studied, indicating that there is coordination among the metal ions and the prepared ligand. A conductivity and magnetics, an elementary micro-analysis and the metal ion percentage were identified. The geometrical structure of the co(II), Ni(II) and Cu preparations depends on the results of these projects (II) and they are octahedral. The mole-ratio (M:L) is (1:2). The conductivity measurements show non-electrical properties, for all prepared complexes.The final stage involved the study of the biological activity of prepared component to two type of pathogenic bacteria: (G+) Staph. aureus and Escherichia coli (G-) using Well diffusion methods .Three different concentration were tested  (100 ,500, 1000) ppm in Ethanol Absolute(99%) as a solvent . The prepared compounds showed different results (high, moderate, low, and inactive).