Introduction: Breast cancer is the most common cancer. In the course of cancer progression, there are specific neuromuscular disturbances that directly affect coordination. Hand-writing is regarded as a vital constituent when it comes to the tracing of neuromuscular coordination. Aim of the study:The study aimed to study handwriting analysis in female cancer breast patients trying to find simple useful, non-invasive ways to help in early detection, diagnosis, and follow-up.Subjects and methods: 160 participants were included in the study. Participants were asked to write the same text (two Arabic sentences and her name in the Arabic language) and draw lines on another paper, and then all handwriting samples were scanned and examined. Results and Conclusion:Our study shows that many handwriting changes such as Tremor, wide spaces between words, heavy writing pressure, slope or slant of writing upward slant or down slant, and heaviness of Initial and terminal strokes were observed. These changes were in samples of the patient received multiple lines of treatment, in patients with metastasis, or had another associated type of tumor such as ovarian, bladder cancers or lymphoma.