A seven-year-old girl, whose right knee gave way, was found to have anterolateral instability of the joint associated with congenital aplasia of the anterior cruciate ligament. Instability was present in the final 30 degrees of extension, and has been controlled by a brace which prevented this movement.

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Sera from a reference pool, 29 controls and 18 patients with Graves' disease were fractionated on sepharose CL-4B protein A. After a primary elution of the unbound fraction at normal pH, IgG subclasses 1, 2 and 4 were eluted using a discontinuous pH gradient. The eluate was monitored by UV-absorption at 280 nm and three peaks were observed within narrow pH ranges in all sera tested. From the reference pool (n = 10)

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