An enzymatic, fluorometric method is described for determination of serum urea on silicone-rubber pads. In this method, the reagents are lyophilized on the surface of the pads, NADH on one side and a mixture of urease, glutamate dehydrogenase, and α-ketoglutarate on the other. The rate of disappearance of NADH fluorescence at 460 nm (excitation wavelength, 340 nm) is monitored and related to serum urea concentration. The calibration curve is linear to 250 mg of urea per liter. The method affords a rapid, simple, and inexpensive means for urea assay, the results of which correlate well with automatic diacetyl monoxime method (correlation coefficient, 0.998).

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A "reagentless" fluorometric method is described for the analysis of serum creatine kinase (CK) activity. The method is based on the use of silicone rubber pads, upon which are placed all the reagents for assay of CK. The rate of formation of NADH fluorescence at 460 nm is measured and equated to CK activity. The method is simple, rapid, inexpensive, and as little as 3 µl of serum is needed.

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