Sensitive and accurate detection of highly contagious virus is urgently demanded for disease diagnosis and treatment. Herein, based on a multifunctional aggregation-induced emission luminogen (AIEgen), a dual-modality readout immunoassay platform for ultrasensitive detection of viruses has been successfully demonstrated. The platform is relied on virions immuno-bridged enzymatic hydrolysis of AIEgen, accompanying with the in situ formation of highly emissive AIE aggregates and shelling of silver on gold nanoparticles. As a result, robust turn-on fluorescence and naked-eye discernible plasmonic colorimetry composed dual-signal is achieved. By further taking advantage of effective immunomagnetic enrichment, EV71 virions, as an example, can be specifically detected with a limit of detection down to 1.4 copies/μL under fluorescence modality. Additionally, semiquantitative discerning of EV71 virions is realized in a broad range from 1.3 × 10 to 2.5 × 10 copies/μL with the naked eye. Most importantly, EV71 virions in 24 real clinical samples are successfully diagnosed with 100% accuracy. Comparing to the gold standard polymerase chain reaction (PCR) assay, our immunoassay platform do not need complicated sample pretreatment and expensive instruments. This dual-modality strategy builds a good capability for both colorimetry based convenient preliminary screening and fluorescence based accurate diagnosis of suspect infections in virus-stricken areas.
The chemistry of transition metal-containing metallabenzenes has attracted considerable attention.[1] Previous studies have led to the isolation and characterization of a number of stable metallabenzenes, especially those of osmium, [2][3][4] iridium, [5][6][7][8] platinum, [9] ruthenium, [10,11] and rhenium. [12] Many interesting chemical properties of metallabenzenes have also been discovered. For example, it has been demonstrated that metallabenzenes can undergo electrophilic substitution reactions, [2a, 5d] cycloaddition reactions, [8d, 9c] nucleophilic addition reactions, [13] and nucleophilic aromatic substitution of hydrogen. [14] Another common reactivity of metallabenzenes is that they can undergo migratory insertion reactions to give cyclopentadienyl complexes. The transformation has been demonstrated with well-characterized metallabenzenes [2e, 6b,-d,e, 10b] as well as a spectroscopically characterized ruthenabenzene, [15] and it has been proposed as a key step in the formation of cyclopentadienyl complexes.[1]Compounds closely related to metallabenzenes are metallabenzynes.[16] Compared with the chemistry of metallabenzenes, that of metallabenzynes is much less developed, which is partly due to the lack of convenient methods to synthesize such compounds.[17] Structurally, metallabenzynes are similar to metallabenzenes in that both have a delocalized structure. Thus it might be expected that metallabenzynes should have properties similar to those of metallabenzenes. Indeed, previous studies have demonstrated that metallabenzynes, like metallabenzenes, can also undergo electrophilic substitution reactions [18] and nucleophilic addition reactions.[17c] As formation of cyclopentadienyl complexes from metallabenzenes by migratory insertion reactions is well-known, one might expect that metallabenzynes could also undergo migratory insertion reactions to give carbene complexes. However, such reactions have not been previously observed. Herein, we present a reliable method to prepare osmabenzynes along with the first examples of conversion of metallabenzynes into carbene complexes.We recently observed that reaction of zinc with the osmium vinyl carbyne complex [OsCl 3 { C À CH = C(2-ClC 6 H 4 ) 2 }(PPh 3 ) 2 ] produced a osmanaphthalyne complex. [19] The reaction was proposed to proceed through a 16e fourcoordinate square planar osmium carbyne complex, which undergoes an oxidative addition reaction involving a CÀCl bond. Inspired by the observation, we envisioned that reactions of zinc with carbyne complexes of the type [OsCl 3 { C À CH = CR À CR' = CClR''}(PPh 3 ) 2 ] might lead to the formation of new osmabenzynes.To test this hypothesis, we first prepared the meridional osmium carbyne complex 1 and then treated it with zinc in THF at room temperature. An in situ 31 P NMR study showed that osmabenzyne 2 with substituents on the C3 and C5 positions was produced as the major phosphorus-containing product (Scheme 1), which can be isolated in 62 % yield after column chromatography.The osmabenzyne 2 has bee...
Three new diterpenoids, yuexiandajisu D (1), E (2) and F were isolated from the roots of Euphorbia ebracteolata, along with eight known diterpenoids, jolkinolide B (4), jolkinolide A, ent-11alpha-hydroxyabieta-8(14),13(15)-dien-16,12alpha-olide (6), ent-(13S)-hydroxyatis-16-ene-3,14-dione, ent-3beta,(13S)-dihydroxyatis-16-en-14-one, ent-3-oxokaurane-16alpha,17-diol, ent-16alpha,17-dihydroxyatisan-3-one and ent-atisane-3beta,16alpha,17-triol. The structures of all compounds were deduced using spectroscopic methods and confirmed for 1 and 2 by single-crystal X-ray diffraction. A biogenetic pathway for the formation of 1 and 2 is proposed briefly. Cytotoxic activities were evaluated against ANA-1, B 16 and Jurkat tumor cells. Jolkinolide B (4) displayed modest activity on ANA-1, B 16 and Jurkat tumor cells with IC50 values 4.46 x 10(-2), 4.48 x 10(-2), 6.47 x 10(-2) microM, and ent-11alpha-hydroxyabieta-8(14),13(15)-dien-16,12alpha-olide (6) showed significant activity against ANA-1 and Jurkat cells with IC50 values 7.12 x 10(-3) and 1.79 x 10(-2) microM. Compound 1 was found to be slightly active against ANA-1 cells with an IC50 value 2.88 x 10(-1)microM. Structure-activity relationships of isolated compounds are also discussed.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2025 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.