Waning of vaccine protection was observed among individuals aged 65years old or over in two A(H3N2)-dominant influenza seasons.

No abstract

Neutrophils and subsets of lymphocytes bind to E-selectin, a cytokine inducible adhesion molecule on endothelial cells. The E-selectin-ligand-1 (ESL-1) is a high affinity glycoprotein ligand which participates in the binding of mouse myeloid cells to E-selectin. The sequence of mouse ESL-1 is highly homologous to the cysteine rich FGF receptor (CFR) in chicken and the rat Golgi protein MG160. We have analysed the subcellular distribution of ESL-1 by indirect immunofluorescence, flow cytometry, various biochemical techniques and by immunogold scanning electron microscopy. We could localize ESL-1 in the Golgi as well as on the cell surface of 32Dc13 cells and neutrophils. Cell surface staining was confirmed by cell surface biotinylation and by cell surface immunoprecipitations in which antibodies only had access to surface proteins on intact cells. In addition, ESL-1(high) and ESL-1(low) expressing cells, sorted by flow cytometry, gave rise to high and low immunoprecipitation signals for ESL-1, respectively. Based on immunogold labeling of intact cells, we localized ESL-1 on microvilli of 32Dc13 cells and of the lymphoma cell line K46. Quantitative evaluation determined 80% of the total labeling for ESL-1 on microvilli of K46 cells while 69% of the labeling for the control antigen B220 was found on the planar cell surface. These data indicate that ESL-1 occurs at sites on the leukocyte cell surface which are destined for the initiation of cell contacts to the endothelium.

Volhard. He, as well as Epstein,7 repeatedly pointed to a general metabolic disturbance, perhaps of the ductless glands, as a cause of this disease, and that these cases do not seem to be primarily a disease of the kidney. Symptoms: Onset.-In some of our cases there was a history of pallor or a generally subnormal condition existing for some months previously. In a few cases there was a distinct history of malaise, vomiting and headache; in the majority of cases, however, the first symptom noticed by the parents, was the swelling about the face, the edema increasing with great rapidity. In ten cases the edema began in the face and within twenty-four hours the legs were involved. In other cases the edema of the legs and face occurred simultaneously, and in the course of about a week the other subcutaneous tissues were also involved.Temperature.-Temperature elevation was not usitaiiy present, and the children were not acutely ill ; the throat was generally in fairly 6.

No abstract