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Three inter-generic hybrid strains were obtained by pairing compatible neohaplonts recovered by dedikaryotization of four parental strains, i.e. Pleurotus ostreatus (CC060), Pleurotus djamor (CC051) and Lentinula edodes (CC003 and CC004). Fifteen neohaplonts were recovered employing homogenization time periods for Pleurotus spp. since 60 to 90 s, and for Lentinula edodes strains time periods ranged from 5 to 20 s, and incubation at 28 °C in a peptone-glucose solution (PGS). The mycelial growth of the parental and hybrid strains was determined on malt extract agar (MEA) and wheat grain by calculating the parameters A, B and C of nonlinear regression models. For the parameter A, the mycelial growth on MEA showed values ranged from 1.08 to 1.28, and for the parameter B since 5.65 to 20.85. On the other hand, the A values by mycelium growth on wheat grain ranged from 1.57 to 16.13, for B since 28.36 to 86.53 and for C were in an interval from 11.89 to 44.24. Parental and hybrid strains presented instantaneous velocity values on MEA since 2.88 to 7.26 cm2•day-1, whereas on wheat grain were in an interval from 5.45 to 10.05 cm3•day-1. Furthemore, the µmax values on MEA and wheat grain were calculated by using the Gompertz model and the λ values on both medium were estimated by using the Hill model, the µmax values on MEA ranged from 0.26 to 1.69 day-1 and λ values in an interval from 0.41 to 2.74, whereas the µmax values on wheat grain ranged from 0.05 to 0.43 day-1 and λ since 1.82 to 28 h. Moreover, the estimated equations based on nonlinear models were used to calculate the µmax values on MEA and wheat grain of the strains and the λ values were obtained by using the µmax values of the proposed equations on the Hill model, the µmax values on MEA ranged from 0.44 to 1.27 day-1 and the λ values between 0.57 to 1.72 h, while the µmax values on wheat grain ranged from 0.04 to 0.64 day-1 and λ values in an interval from 1.26 to 20.11 h. The results evidenced that the hybrid strains of PleurotusxLentinula presented highest rate of growth in comparison with the parental, encouraging the production of hybrid strain and their use in the industrial field.

Production of hybrid strains is accomplished by mating monosporic isolates or neohaplonts, obtained either by chemical dedikaryotization or by production of protoplast. However, differences in growth rate among recovered neohaplonts have been reported. The presence of phenotypic and genetic changes among the neohaplonts recovered either by chemical dedikaryotization or by production of protoplast, was evaluated by measuring growth and morphology, and by molecular characterization using six ISSR markers to identify polymorphisms. Neohaplonts recovered by both methods presented variation in growth rate depending on their compatibility type and recovery method. Using ISSR markers, 59.2% polymorphism was established. Neohaplonts recovered by both monokaryotization procedures presented differences in growth rate and polymorphism.

Introduction: Ganoderma lucidum is one of the most worldwide known medicinal mushrooms. However, this species of mexican mushroom has notable differences compared to its similar one since it has not been studied enough so it is important to determine its characteristics once it has been cultivated and its extracts have obtained in a standardized form.Method: The polysaccharides of the extracts of Ganoderma lucidum strain CP-145 were measured by the quantum magnetic resonance analyzer MARSIII of Bruce Copen (MARSIII), a proposed technique to estimate values of compounds in the agricultural area, these results were compared with two standardized techniques: Fehling method of sugar reducers and Gas Chromatography - Mass spectrometry (GC-MS), the extracts to be measured were obtained from the traditional culture of basidiocarps (control) and extract of basidiocarps added with acetylsalicylic acid (ASA).Results: The results for the MARSIII quantum magnetic analyzer were 4.967 ± 1.016 and 8.110 ± 1.416% in control extract and extract added respectively, by the Fehling´s method 8.784 ± 2.019% and 41.326 ± 1.430% in control extract and extract added respectively, for CG- MS 7.050 ± 1.527 and 18.456 ± 2.937% in control extract and extract added respectively.                      Discussion and Conclusion: Significant differences were found on the Fehling´s techniques and on the GC-MS for both extracts, but not in the MARSIII analyzer detection, which indicates that it is not an adequate method to detect variations in the concentrations of polysaccharides in this type of extracts.