The objective of this study was to determine the prevalence of canine brucellosis (Brucella canis) in two districts of the province of Callao. A total of 456 sera were collected from dogs without distinction of breed, sex and age. Samples were analyzed by the Agar Gel Immunodiffusion (AGID) test using B. ovis antigen. The results indicated that 15.6 ± 3.3 (71/456) of sampled animals were positive. No statistical differences were found due to district, breed, sex or age; however, differences were found (p<0.05) beetween dogs with and without reproductive history (26.5 and 8.6% respectively).