Background: Fatigue is one of the risks of a decrease in the health status of the workforce. The risks that can be caused due to fatigue include a decrease in work motivation, low performance, low quality of work, many errors in work, low work productivity, causing work stress. Stress is a situation of emotional tension and pressure experienced by someone when facing many demands and obstacles that can affect one's emotions, thoughts and physical condition. Objective: The purpose of this study was to determine the relationship of work stress, age and years of work with subjective fatigue in the production department employees at PT. Sunrise Bumi Textiles, Bekasi City. Method: This study uses an analytical method with Cross sectional design. The number of samples is 57 people at the time of the entire shift. The sampling technique uses Proportional Random Sampling and Chi Square Test as statistical tests. This study used the IFRC (Industrial Fatigue Research Committee) questionnaire and DASS 21 (Depression Anxiety and Stress Scale). Result: The results of the study revealed that there was a significant relationship between work stress and subjective fatigue p value = 0.0001. There is no relationship between age and subjective fatigue p value = 0.15, and there is no significant relationship between tenure and subjective fatigue p value = 0.263.Conclusion: Workers who suffered from work stress more easily become fatigue. Recommendation from this research is: conduct worker placement according to age and years of service, provide a control room to rest, provide drinking water at work, conduct training or training for new workers.