The production of fermentable sugar from sugarcane bagasse acid hydrolysis method was investigated in this study. Experimentation on the determination of acid for hydrolysis was carried out to identify the suitable type of acid for the effective hydrolysis process. The results revealed that sulphuric acid (H2SO4) is a better hydrolysing agent than phosphoric acid (H3PO4). This study also analysed the impact of temperature, on the yield of fermentable sugar between the sugarcane bagasse. The highest amount of fermentable sugar of 10.26 g/L was produced by sugarcane bagasse at temperature of 90°C, acid concentration of 4% (w/v) and a reaction time of 3 hours.