Doxorubicin is a chemotherapeutic agent that causes a lot of side effects in high doses. Thus, combination with a co-chemotherapeutic agent which can increase its toxicity on cancer cells, is needed to reduce its therapeutic dose. Red betel leaves (Piper crocatum Ruiz & Pav.) have been known to contain flavonoids and alkaloids that have anticancer activity. This study was conducted to determine the cytotoxic effect and apoptosis induction of red betel leaves methanolic extract (RBM), doxorubicin (dox) and the combination of them on WiDr cells as model of colon cancer. RBM was extracted by soxhlet method using methanol. Cytotoxicity assay was performed using MTT assay for both single and combination treatments for 24 hours to determine IC50 and CI as their parameters. Apoptosis induction was analyzed by double staining method using ethidium bromide-acridine orange staining. Treatment of RBM and dox on WiDr cells for 24 hours showed cytotoxic activity with IC50 100 μg/mL and 1.6 μM respectively. Combination of RBM and dox performed synergism effect with CI<0.9 (p<0.05). Combination of RBM (12.5 μg/mL) and dox (0.4 μM) increased the number of apoptosis cells compared to each single treatment. Based on this study, it can be concluded that red betel leaves methanolic extract is potential to be developed as a co-chemotherapeutic agent of doxorubicin on colon cancer but still need further study to disclose the underlying molecular mechanisms.Keywords :  Red betel leaves (Piper crocatum Ruiz & Pav.), doxorubicin, WiDr cells, co- chemotherapeutic agent

Breast cancer is one type of cancer with a high mortality rate due to its metastatic property. Red betel leaves (Piper crocatum Ruiz dan Pav) has been known as herbal medicine containing biophenolic, such as apigenin and luteolin derivatives which has cytotoxic activity toward cancer cells. This study is intended to explore the inhibitory effect of Piper crocatum leaves methanolic extract (PCM) on cell proliferation and migration by using 4T1 cells as model of metastatic breast cancer. By using MTT assay, PCM performed cytotoxic activity in a dose dependent manner with IC50 value of 120 μg/mL. Wound healing assay revealed that migration inhibitory activity of PCM on 4T1 cells at the concentration of 30 µg/mL. In conclusion, PCM perform cytotoxic effect and antimigration activity toward metastatic breast cancer cells.Keywords : breast cancer cells, Piper crocatum Ruiz & Pav, cytotoxic, cell mgration

After the implementation of halal product assurance (HPA) became a public policy, the assurance for halal pharmaceutical products is a must. However, the implementation has been relaxed for several years. This shows that the policy implementation is complicated. This study aimed to analyze and evaluate the implementation process of the HPA policy for pharmaceutical products in Indonesia. We applied Van Meter Van Horn’s theory using the qualitative method through in-depth interviews with eight informants and a review ten documents. The result shows that the implementation is going well but is still not optimal, especially from the point of view regarding halal certification for pharmaceutical products. The main obstacle for business actors in this implementation is obtaining halal raw materials because most of them are still imported. Moreover, a lack of massive socialization and education have also appeared. All stakeholders involved should improve performance according to their respective duties to implement better when the obligation has begun.

The coronavirus disease 2019 (COVID-19) pandemic, caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was declared a public health emergency in many countries, including Indonesia. The World Health Organization (WHO) released an educational video, entitled "How to Protect Yourself Against COVID-19", which explained how to prevent the spread of COVID-19. This study is a qualitative study that aims to pre-test this video, based on the Information, Education, Communication (IEC) theory, by analyzing teenagers’ perceptions of the video. The design used was a Rapid Assessment Procedure (RAP), and the data were collected by conducting in-depth interviews with participants, through virtual meetings. The data were analyzed using a qualitative data matrix and thematic analysis. The results showed that all participants found the video regarding the prevention of COVID-19 spread to be attractive and comprehensible. In addition, the participants felt that the contents of the video were not contradictory to their personal values and that the message was targeted to everyone, including themselves. The video was also perceived to be persuasive. Overall, the participants held a generally positive perception of the video.

Coronavirus disease-2019 (COVID-19) encourages the community to prioritize basic and personal health needs. This study aimed to explore the consumer behaviour of traditional medicine and health supplements during the COVID-19 pandemic in Indonesia using the Consumer Decision Model approach. A quick online cross-sectional survey involving 215 respondents was carried out from December 23, 2020, until January 2, 2021. This study revealed that the majority of respondents consume the products as forced by the pandemic situation, and their use has increased than before the pandemic. The primary reason for consuming the product is to increase their immunity during a pandemic, and they felt more healthy after consuming it. Social media and television are the primary sources of product information. Furthermore, we found that the majority of respondents were branded product consumers. Respondents were more likely to have good behaviour based on knowledge of the products' legality, dosage, indication, instructions for use, and expiration date. This study sets appropriate consumer behaviour and performs the statistical tests only for branded product consumers. It is concluded that consumer behaviour in this study is related to product advertisement exposure and acceptance, environmental influence, and individual difference.