An efficient protocol of shoot organogenesis and plant regeneration from internode derived callus has been developed for Capsicum annuum. Optimal callus was developed from internodal segments on Murashige and Skoog (MS) medium supplemented with 10 μM 2,4-dichlorophenoxy acetic acid (2,4-D) and 2.0 μM 6-benzyladenine (BA). Shoot differentiation was achieved from the surface of callus when transferred on shoot induction medium containing BA and thidiazuron (TDZ) alone or in combination. The highest number of de novo adventitious shoots (25.4±1.42) and shoot length (4.6± 0.37 cm) was recorded on MS medium supplemented with 5.0 μM BA and 2.5 μM TDZ. The individual elongated shoots were rooted well on MS medium supplemented with 1.0 μM Indole-3-butyric acid (IBA). The in vitro raised plantlets with properly developed shoot and roots were acclimatized successfully and grew well in the greenhouse. All the regenerated plants appeared normal with respect to morphology and growth characteristics with 85% survival rate.

Bambusa balcooa.Roxb. is one of the most commercially important bamboo species. Some varieties of bamboo are being grown in the company's green house. However this species is very rare and it was not cultured until now due to lack of demand and maintenance problems. In this work B. balcooa ex-plants have been established and propagated by the axillary shoot bud proliferation method. Earlier several works have been done on this plant and a protocol has been. This work emphasizes on effects of phytohormones at different concentrations and combinations upon the novel propagules that develop from the bamboo explants on placing them in multiplication media. The propagules placed in media containing cytokinins BAP (2 mg/L) and Kinetin (1 mg/L) exhibited best results of linear as well as radial growth. The other propagules in media with combinations of BAP, Kinetin and NAA were found to be dried out and did not depict noticeable growth. Comparatively next best growth pattern to the former was observed in control media that lacked phytohormones. This experiment was helpful in estimating the quantity and effect of particular plant growth regulating hormones on the Bambusa balcooa.Roxb.

Bambusa balcooa.Roxb. is one of the most commercially important bamboo species. Some varieties of bamboo are being grown in the company’s green house. However this species is very rare and it was not cultured until now due to lack of demand and maintenance problems. In this work B. balcooa ex-plants have been established and propagated by the axillary shoot bud proliferation method. Earlier several works have been done on this plant and a protocol has been. This work emphasizes on effects of phytohormones at different concentrations and combinations upon the novel propagules that develop from the bamboo explants on placing them in multiplication media. The propagules placed in media containing cytokinins BAP (2 mg/L) and Kinetin (1 mg/L) exhibited best results of linear as well as radial growth. The other propagules in media with combinations of BAP, Kinetin and NAA were found to be dried out and did not depict noticeable growth. Comparatively next best growth pattern to the former was observed in control media that lacked phytohormones. This experiment was helpful in estimating the

Potassium permaganate has been used since ages for disinfection of water. It is known for oxidation of cell membrane of micro organisms. But the pink colour prevailing after use makes people reject the water on aesthetic grounds. Water samples from bore wells and tap water from public places were collected from different areas of Visakhapatnam. Physio-chemical and microbial analysis analyses of the water samples were carried out. A dose based permaganate treatment of water depending upon number of colony forming units and species of microorganism present in the water was carried out [1,2,5]

No abstract