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Nanoscale secondary ion mass spectrometry analysis of the cross-sections of virgin black hair and bleached hair indicated that the oxygen content in melanin granules was increased by bleach treatment.

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Background/purpose: In oxidative coloring, the hair cuticle layers are not only the penetration pathway for active ingredients but also one of the most important dyeing regions. The dyeing mechanism of oxidative dyes in fine structures of the cuticle remains unclear. To investigate the dyeing behavior of oxidative dyes in fine structures of the cuticle, hair cross-sections were analyzed by nanoscale secondary ion mass spectrometry (NanoSIMS). Methods: The preparation method of hair cross-section for NanoSIMS measurement was improved. Improved hair crosssections were analyzed using NanoSIMS. Results: The cuticle layer thickness of the hair cross-section could be widened. It was confirmed that 12 C À ions were more strongly detected from endocuticle than from other fine structures of cuticle. The NanoSIMS 12 C À image and hue saturation intensity (HSI) D À / 1 H À ratio image of the hair, dyed with

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