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A K+ doped glass slab optical waveguide (SOWG) was coated with octadecylsilane (ODS) to separate and concentrate analytes onto the SOWG with hydrophobic interaction between analytes and ODS. As an absorption detector of flow analysis, the ODS–SOWG detector was almost insensitive to a blue dye (bromothymol blue) which is not adsorbed to the surface of the SOWG. On the other hand, it was highly sensitive to another blue dye of coomassie brilliant blue G-25O (CBB) which is firmly adsorbed onto the SOWG surface.

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