An extremely UV-resistant stage was found to appear only temporarily in the early phase of germination of B. subtilis spores. Kinetic analysis of the course of germination with special reference to ultraviolet and heat resistance revealed that the germinating spores remain at this transient stage for one to six minutes depending on the condition of germination, and Transient UV-resistant Stage in Spore Germination 281

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The viability of freeze-dried Lactobacillus bulgaricus B-1 was affected by rehydration temperature, and maximum recovery of the viable cells was obtained when they were rehydrated at 20 to 25°C. Cellular ribonucleotides leaked out from the freeze-dried cells during rehydration, but there was no correlation between the viability of cells and the amount of leaked substances. Rehydration of the freeze-dried cells in the presence of RNase caused marked loss of viability. These results suggest that the cell surface was damaged by freeze-drying and its selective permeability was lost to some extent.