rOIT induced desensitization to egg and thus improved the QOL of guardians; however, the participants experienced frequent allergic reactions due to the treatment.

No abstract

Our standard curves could be useful for diagnosis and control evaluation of childhood asthma.

These results suggest that IFN-γ represses MUC5AC expression, disturbing binding of Sp1 to its target sequences.

Human thymic CD1a؊ CD4 ؉ T cells in the final stage of thymic maturation are susceptible to anergy induced by a superantigen, toxic shock syndrome toxin-1 (TSST-1). Thymic CD4؉ T-cell blasts, established by stimulating human thymic CD1a ؊ CD4 ؉ T cells with TSST-1 in vitro, produce a low level of interleukin-2 after restimulation with TSST-1, whereas TSST-1-induced adult peripheral blood (APB) CD4؉ T-cell blasts produce high levels of interleukin-2. The extent of tyrosine phosphorylation of the T-cell receptor chain induced after restimulation with TSST-1 was 2-4-fold higher in APB CD4؉ T-cell blasts than in thymic CD4 ؉ T-cell blasts. The tyrosine kinase activity of Lck was low in both thymic and APB CD4 ؉ T-cell blasts before restimulation with TSST-1. After restimulation, the Lck kinase activity increased in APB CD4؉ T-cell blasts but not in thymic CD4 ؉ T-cell blasts. Surprisingly, Lck was highly tyrosine-phosphorylated in both thymic and APB CD4 ؉ T-cell blasts before restimulation with TSST-1. After restimulation, it was markedly dephosphorylated in APB CD4 ؉ T-cell blasts but not in thymic CD4؉ T-cell blasts. Lck from APB CD4 ؉ T-cell blasts bound the peptide containing the phosphotyrosine at the negative regulatory site of Lck-505 indicating that the site of dephosphorylation in TSST-1-activated T-cell blasts is Tyr-505. Confocal microscopy demonstrated that colocalization of Lck and CD45 was induced after restimulation with TSST-1 in APB CD4؉ T-cell blasts but not in thymic CD4 ؉ T-cell blasts. Further, remarkable accumulation of Lck in the membrane raft was observed in restimulated APB CD4 ؉ T-cell blasts but not in thymic CD4؉ T-cell blasts. These data indicate that interaction between Lck and CD45 is suppressed physically in thymic CD4 ؉ T-cell blasts and plays a critical role in sustaining an anergic state. Human thymic CD1aϪ CD4 ϩ T cells in the final stage of maturation (1) and cord blood CD4 ϩ T cells are susceptible to anergy induction by in vitro stimulation with a superantigen, toxic shock syndrome toxin-1 (TSST-1).