A sensitive method for acetylcholinesterase (AChE) histochemistry has been developed which permits simultaneous observation of fme fiber processes and neuron cell bodies. In rat brain, distinctive configurations can be observed which have been difficult to see by other techniques. Ihe staining procedure involves two steps. Tissue sections are incubated first in Karnovsky and Roots medium diluted one-hundredfold; and then with a mixture containing diaminobenzidine (DAB) and H202. The reaction product of the first step induces deavage of hydrogen peroxide in the second step, with a resulting oxidation of DAB to yield a
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