During the course of screening for inhibitors of trehalase, we found the pseudodisaccharide termed trehazolin1 (1) in a culture broth of Micromonospora, strain SANK 62390n. This paper describes the isolation, structure determination, and inhibitory activity of trehazolin. One loopful of the producing organism was aseptically transferred from an agar slant into baffled 500-ml Erlenmeyer flasks each containing 80ml of a primary seed medium.This mediumwas composed of glucose 1%, glycerol 1%, oatmeal 0.5%, sucrose 1%, soybean meal 2%, Casamino acids 0.5%, pressed yeast 1%, CaCO3 0.1%, and Disfoam CB-442 (Nippon Yushi Co.) 0.01%. After formulation, the pH was adjusted to 7.0 with aq NaOH, and the mediumwas sterilized. The incubation was performed on a rotary shaker at 220rpmat 28°C for 216 hours. Forty ml of the primary seed culture were aseptically transferred into baffled 2-liter Erlenmeyer flasks each containing 800ml of a secondary seed/production medium which was composed of glucose 2%, soluble starch 1 %, pressed yeast 0.9%, meat extract 0.5%, Polypepton 0.5%, NaCl 0.5%, CaCO3 0.3%, and Disfoam CB-442 0.01%. The medium was then adjusted to pH 7.2

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