Many studies show that hyperglycemia can lead to injury to the intestinal mucosal barrier, increasing the likelihood of infection, leading to intestinal inflammation in people with type 2 diabetes. Recently, investigations indicate that the gut microbiome plays a very important role in this disease in which Lactobacillus in the gut has been shown to regulate glucose and lipid metabolism, improving oxidative stress, and inhibiting inflammatory responses. In this study, by the method of oriented isolation, the number of Lactobacillus bacteria in the subgroup of healthy people was determined to be (3,9 ± 1.2) x 103 CFU/g and in the group of diabetic patients was (2.7 ± 0.8) x 103 CFU/g. The study identified 17/68 selected Lactobacillus strains that were resistant to both Staphylococcus aureus ATCC13709 and Escherichia coli ATCC 11105. Moreover, these strains were also resistant to acid pH 2. Besides that, 16/17 strains tested were able to survive over 90% in 0.3% bile salt environment. Among the 17 strains studied, 3 strains of Lactobacillus 13, Lactobacillus 16, Lactobacillus 17 showed probiotic characteristics such as antibacterial ability, acid tolerance and bile salt tolerance. All three strains of Lactobacillus 13, Lactobacillus 16, and Lactobacillus 17 belong to species of Lactobacillus plantarum. This result makes a useful contribution to guide the application of Lactobacillus strain in creating dietary supplements for people with type 2 diabetes.

Lactobacillus sp. in the digestive tract are capable of producing H2O2 to inhibit the growth of harmful bacteria and balance the gut microflora. In this study, we have isolated 115 strains of Lactobacillus spp. from stool samples of healthy people in Ha Noi. Of the 50 tested Lactobacillus strains, 9 strains were capable of producing H2O2, of which the Lac.VFE-14 strain produced highly H2O2 with a concentration of 2.183 mM, followed by Lac.VFE-08 strains (2.081 mM) and Lac.VFE-04 (2.067 mM). All three strains grew well in MRS medium supplemented with bile salts or adjusted to low pH value. With 0.3% of bile salt, the survival rates of these 3 strains were 99%, 95% and 97%, respectively. At pH 3.0, after 3 hours of cultivation, the survival rates of the three strains were 98.54%, 94.15% and 95.27%, respectively. In addition, each of the cell-free culture supernatants of these three strains that inhibit the growth of S. aureus ATCC-23235. The inhibition zone diameters of the three strains were 19.0±1.0 mm, 14.0±1.0 mm and 11.7±1.3 mm, respectively. The results of 16S rRNA gene analyses showed that Lac.VFE-14, Lac.VFE-08 and Lac.VFE-04 had high similarity scores with L. plantarum ZZU 23 (100%), L. rhamnosus JCM 1136 (99%) and L. plantarum S7 (98.65%), respectively. This study indicates that all three strains have the potential to be used as probiotics in the future.

Objectives: To evaluate the outcome of selective reconstructive anterior cruciate ligament (ACL) surgery using the All-inside technique with a four-strand semitendinosus or gracilis autograft tendon. Subject and methods: 34 patients were diagnosed with a partial tear of the ACL and underwent selective reconstructive surgery at the Orthopedic Center, Military Hospital 103 from November 2018 to September 2020. Lysholm knee scale and IKDC class were used to access post-operatively clinical outcomes. Stability was evaluated using the anterior drawer test, the Lachman test, the Pivot shift test, and the Rolimeter measure at 20o of knee flexion. Results: The pre-operative and post-operative Lysholm scores were 49.5 ± 18.4 and 94.47 ± 6.81, respectively (p < 0.001).         Pre-operatively, most patients were classified as C and on the IKDC score. At the final follow-up, IKDC scores included 22 class A, 10 class B, 2 class C (p < 0.001). The pre- and post-operative laxity measured by Rolimeter were 5.15 ± 1.5 mm and 1.74 ± 1.34 mm, respectively (p < 0.001). Pre-operatively and post-operatively, the Pivot-shift was absent in all cases. Conclusion: Selective reconstruction of the injured ACL bundle restores knee stability and function. All-inside technique can be used to reconstruct the rupture ACL bundle with many advantages. Longer follow-up is needed to fully evaluate clinical outcomes after selective reconstruction in partial ACL tears. * Keywords: All-inside; Partial tear; Selective reconstruction.