A study of the relationship of the raw wastewater BOD 5 :cBOD 5 ratio was conducted at three wastewater treatment plants across the United States. Gwinnett County, Georgia; King County, Washington; and Littleton-Englewood, Colorado conducted a 3-month study using side-by-side samplers. The purpose of the study was to determine if cleaning wetted sampler parts routinely affected the ratio. One of the samplers was cleaned twice a week and the other was not cleaned. The results of the study show that cleaning the wetted parts of the sampler has a small but statistically insignificant impact on the BOD 5 :cBOD 5 ratio. The study also showed a potential relationship of wastewater temperature to the BOD 5 :cBOD 5 ratio, and also the possible influence of sampler type on raw wastewater characterization.

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Gwinnett County is in the Piedmont region of Georgia where streams have finite assimilative capacity. Gwinnett County is also upstream of water supply and recreational waters for much of the Atlanta metropolitan region. This headwaters geography has required the county to adopt advanced water reclamation methods to keep pace with increased demand for wastewater service.