[FeFe]-hydrogenases are known for their high rates of hydrogen turnover, and are intensively studied in the context of biotechnological applications. Evolution has generated a plethora of different subclasses with widely...

A semi-synthetic screening method for mining the biodiversity of [FeFe]-hydrogenases, expanding the toolbox for biocatalytic H2-gas production.

The mechanism of hydrogen gas formation by [FeFe] hydrogenase is probed under whole cell conditions, revealing the formation of reactive metal hydride species under physiologically relevant conditions.