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A new acetate-requiring mutant was isolated in Neurospora crassa and termed as ace-8. The ace-8 gene is located on the left arm of the linkage group VII near the centromere between thi-3 and qa-3 genes. The ace-8 mutant strain lacks the activity of pyruvate kinase (EC 2.7.1.40). Accumulation of glycolytic intermediates is consistent with the enzyme lesion. The mutant strain is extremely sensitive to glycolytic sugars, glucose and sucrose, but grows well on a medium containing gluconeogenic carbon sources, acetate, ethanol and alanine.

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Cytological and biochemical studies on the red bread mold, Neuro spora crassa, have been carried on by many investigators, and their numerous fruits have been accumulated (Horowitz 1950, Bonner 1951, etc.). On the studies of morphological mutants, however, only their structures and the modes of inheritance have been described and there have been few literatures in which explanation was given on the mechanism of inducing their mutant structures. The present report will add some knowledge to us in such a field.