To develop a simplified method that can rapidly prepare DNA microarray probes in a massive scale, a lambda phage genomic DNA-fragments library was constructed for the microarray-probes collection. Four methods of DNA band recovery from the first PCR products were tested and compared. The DNA microarray probes were collected by a novel method of nested PCR that was mediated by gel isolation of the first PCR products. This method was named GIN-PCR. The probes that were prepared by this GIN-PCR technique were used as subjects to fabricate a DNA microarray. The results showed that a wooden toothpick was superior to the other 3 methods, since this technique can steadily transfer the DNA bands as the template of the second PCR after the first PCR. A group of probes were successfully collected and DNA microarrays were constructed using these probes. Hybridization results demonstrated that this technique of DNA recovery and probe preparation was rapid, efficient, and effective. We developed a cost-effective and less labor-intensive method for DNA microarray probe preparation by nested PCR that is mediated by wooden toothpick transfer of the DNA bands in the gel after electrophoresis.

A gravity compensation algorithm for master manipulator used in minimally invasive robot surgery is proposed in this paper. The Lagrange dynamic equation is used to solve the motor output torque for balancing the master gravity. To avoid time delay in signal processing, multi-thread and multi-event technology are used in software control system. With the algorithm, the force by hand is very small under the condition of exist gravity compensation, less output more than 90% of the external force compared with the lack of gravity compensation.

Enterprise applications office automation system can reduce the burden on staff, improve quality, and promote standardized management, improve the management level, thus promoting informatization construction. This paper launches the research in view of the difficult problems of enterprise OA construction. Firstly, it basics on the construction goal, function structure was studied by the personal office, administrative management, official document system, public information, administrative examination and approval, auxiliary office, system management seven big modules; then, research database platform, through a list of ways describes a list of design components, logic, and so on, Domino database component consisting of access control. Contents of this paper, for improving the level of enterprise information, improve office efficiency and achieve economic aspects of business by leaps and bounds and so will play a positive role in promoting.

Software Quality scheme selection is an important pre-work of software project, For software quality evaluation index are difficult to quantify and has the characteristics of uncertainty, this paper uses a nonlinear SVM classification algorithm research. Firstly, to build a software quality scheme evaluation index system composed of 12 indicators, and through hierarchical structure analysis model representation; Then, research on support vector machine, using graphic to show that the basic idea, through mathematical model to describe the algorithm, constructed Sigmoid kernel function; Finally, to perform case analysis, through 8 have been evaluated schemes to train, drawn 4 unselected scheme evaluation results. This article will apply SVM to software quality scheme selection, it is SVM a new exploration in the field of software engineering, and to improve software quality has an important significance.

A 100W adaptor with universal voltage range 90-265VAC input and 20V/5A output is designed in this paper .The prototype adopted a new level two-stage high power factor power adapter, the PFC(Power Factor Correction) is a Boost converter operating in discontinuous current mode boundary,and the DC/AC using flyback converters. The experimental results showed that the effectiveness of the proposed approach.