Broccoli sprouts are an excellent source of health-promoting phytochemicals such as vitamins, glucosinolates, and phenolics. The study aimed to investigate in vitro antioxidant, antiproliferative, apoptotic, and antibacterial activities of broccoli sprouts. Five-day-old sprouts extracted by 70% ethanol showed significant antioxidant activities, analyzed to be 68.8 μmol Trolox equivalent (TE)/g dry weight by 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulphonic (ABTS) assay, 91% scavenging by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, 1.81 absorbance by reducing power assay, and high phenolic contents by high-performance liquid chromatography (HPLC). Thereafter, sprout extract indicated considerable antiproliferative activities towards A549 (lung carcinoma cells), HepG2 (hepatocellular carcinoma cells), and Caco-2 (colorectal adenocarcinoma cells) using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, with IC50 values of 0.117, 0.168 and 0.189 mg/mL for 48 h, respectively. Furthermore, flow cytometry confirmed that Caco-2 cells underwent apoptosis by an increase of cell percentage in subG1 phase to 31.3%, and a loss of mitochondrial membrane potential to 19.3% after 48 h of treatment. Afterward, the extract exhibited notable antibacterial capacities against Bacillus subtilis and Salmonella Typhimurium with minimum inhibition concentration (MIC) values of 0.39 and 0.78 mg/mL, appropriately, along with abilities against Staphylococcus aureus and Escherichia coli with an MIC value of 1.56 mg/mL. Thus, broccoli sprouts were confirmed as a potential food source for consumers’ selection and functional food industry.

SEA-EU-NET project is based on the program Capacities under the 7th Framework Program (FP7) of the EU Commission with the strategic objective of Integrating and Strengthening the EU-ASEAN Science and Technology Dialogue through coordination and supporting activities. It is a 4 years program started in January 2008, and is currently supported by 22 institutions from Europe and South-East Asia. The objective is to increase the quality, quantity, profile and impact of bi-regional Science and Technology cooperation between the 10 ASEAN countries and the Members and Associated States of the European Union. Every Thematic Priority of FP7 has to implement dedicated international cooperation activities to achieve the program's goals and to address specific problems of 'Third Countries/Regions' (non-EU Members States or non-Associated States). This activity is performed through the organization of annual Thematic Workshops, with the objective to produce a document containing duly justified proposals for Specific International Cooperation Actions. This paper presents the results of the Thematic Workshop on 'Food Quality, Safety and Traceability'. This expert meeting was done in Thailand in February 2009. Titles for 10 possible themes were identified on the following fields: Bacteria antibioresistance, Mycotoxins, Pathogens (bacteria), Pesticides, Heavy metals, Food traceability, Food Supply Chain, Preserving nutrients, Consumers attitudes, Peri-urban markets. (Résumé d'auteur

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