Background. Circular RNAs (circRNAs) may function as the decoys for microRNAs (miRNAs) or proteins, the templates for translation, and the sources of pseudogene generation. The purpose of this study is to determine the diagnostic circRNAs, which are related to lung adenocarcinoma (LUAD), that adsorb miRNAs on the basis of the competing endogenous RNA (ceRNA) hypothesis. Methods. The differentially expressed circRNAs (DEcircRNAs) in LUAD were revealed by the microarray data (GSE101586 and GSE101684) that were obtained from the Gene Expression Omnibus (GEO) database. The miRNAs that were targeted by the DEcircRNAs were predicted with the CircInteractome, and the target mRNAs of the miRNAs were found by the miRDB and the TargetScan. The ceRNA network was built by the Cytoscape. The potential biological roles and the regulatory mechanisms of the circRNAs were investigated by the Gene Ontology (GO) enrichment analysis and the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. The expression of the host genes of circRNAs was examined by the Ualcan. The survival analysis was performed by the Kaplan–Meier plotter. Results. In comparison with normal lung tissues, LUAD tissues contained 7 overlapping cancer-specific DEcircRNAs with 294 miRNA response elements (MREs). Among the 7 DEcircRNAs, 3 circRNAs (hsa_circ_0072088, hsa_circ_0003528, and hsa_circ_0008274) were upregulated and 4 circRNAs (hsa_circ_0003162, hsa_circ_0029426, hsa_circ_0049271, and hsa_circ_0043256) were downregulated. A circRNA-miRNA-mRNA regulatory network, which included 33 differentially expressed miRNAs (DEmiRNAs) and 2007 differentially expressed mRNAs (DEmRNAs), was constructed. These mRNAs were enriched in the biological function of cell-cell adhesion, response to hypoxia, and stem cell differentiation and were involved in the PI3K-Akt signaling, HIF-1 signaling, and cAMP signaling pathways. Conclusion. Our results indicated that 7 DEcircRNAs could have diagnostic value for LUAD. Additionally, the circRNAs-mediated ceRNA network might provide a novel perspective into unraveling the pathogenesis and progression of LUAD.

Objectives/Scope Compared with the underground nature gas storage (UNGS) onshore, the offshore UNGS is further from residential areas and industrial areas, which can shave the peak of natural gas more safely. However, the investment of offshore engineering is higher. Offshore UNGS with reusing offshore depleted gas reservoir construction can not only solves the problems of offshore engineering structure and wells abandonment but also greatly reduces the investment. Methods, Procedures, Process According to the experience of UNGS onshore, the following factors were considered: reservoir buried depth, working gas volume, distance from land, etc. Based on one depleted gasfield in Bohai Bay, the feasibility analysis of an UNGS was carried out, the impact of the offshore UNGS on the environment, the sealing of the underground gas trap and the integrity of the wellbore were evaluated, and the result proves that the sealing of the underground gas trap of the gas field was good. The natural gas pipeline network subsea can provide transportation for the UNGS and compatible to be transformed into the offshore UNGS. However, for one thing, the poor wellbore integrity conditions and imperfect well pattern constraint the reusing, for another, the capacity of offshore facilities need to be improved. Results, Observations, Conclusions According to the economic evaluation, the investment of offshore depleted gas reservoir reusing as UNGS can be reduced by 56% compared with the construction of a new onshore UNGS, and 32% compared with the offshore gas field abandon. A new idea is provided for peak shaving of natural gas, greatly reducing the investment in gas storage construction. Experience has been accumulated by the feasibility analysis of offshore depleted gas field UNGS and that has a bright future. Novel/Additive Information The limitation of natural conditions on project construction, contingency plans for force majeure such as sea ice, storm and earthquake, etc are necessary to be considered, and its implementation still needs the support of the government and relevant non-governmental organizations

The composite photocatalysts revealed the superior photocatalytic performance. A simple hydrolytic way synthesized the BiPO4/Bi4O5I2 composite, which was confirmed by some of characterization methods. The oilfield pollutant of acrylamide was...

This paper uses k-means algorithm to cluster medical imaging disease, and applies Naïve Bayesian classifier to realize the automatic classification of diseases, then achieves hierarchical retrieval of medical imaging examination report information by using .Net technology. It improves the retrieval efficiency and provides convenience for doctors to carry out clinical research