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Insect octopamine (OA) receptors are G‐protein coupled receptors (GPCRs) that play essential roles in physiological and behavioural processes. However, there is little information about the function of OA receptors in the aphids' response to stress. From the genome sequence of Rhopalosiphum padi genome sequence, a cosmopolitan cereal pest, we identified six OA receptor genes RpOAMB, RpOctR, RpOctβ1R, RpOctβ2R, RpOctβ3R, RpOctR‐like with two, one, one, four, four, seven exons, respectively. All the OA receptors contain seven transmembrane domains, which were the signature of GPCRs. Our results showed that (1) the contents of OA increased significantly after food starvation, (2) the transcription levels of RpOAMB, RpOctR, RpOctβ2R and RpOctβ3R increased after starvation and were restored after re‐feeding, and (3) the expression levels of these four genes decreased significantly 48 h post‐injection of dsRNA that targeted the respective genes. Knockdown of RpOctR, RpOctβ2R or RpOctβ3R genes significantly increased aphid mortality under 24 h starvation conditions. Mortality of R. padi injected with dsRpOctR or dsRpOctβ2R was significantly higher than control under 48 h starvation treatments. This is the first report on the role of OA receptors in the starvation response of aphids. The current study provides knowledge for a better understanding the physiological roles of insect OA receptors.

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