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BACKGROUND: A new electrochemical immunosensor for human chorionic gonadotropin (HCG) assay was developed based on Pt hollow nanospheres and silver/titanium dioxide nanocomposites. Silver nanoparticles were initially doped into TiO 2 and chitosan (Cs) colloids to form silver/titanium dioxide nanocomposites (Ag-TiO 2 -Cs), which had good redox electrochemical activity and excellent film-forming ability. Then, the free amino groups of chitosan were used to attach Pt hollow nanospheres for immobilization of human chorionic gonadotropin antibody (anti-HCG) to construct an immunosensor.

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