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Numerous fillers are increasingly used for augmenting volume loss and ameliorating facial wrinkles. Collagen stimulants are the most recent next-generation dermal fillers capable of inducing neocollagenesis. To investigate biophysical characteristics, the safety and efficacy of the newly developed combination, poly(L-lactide) or polyl-lactic acid (PLLA) and poly(lactide-co-glycolide) (PLGA), were compared with those of poly-para-dioxanone (PPDO), poly-l-lactic acid (PLLA), and polycaprolactone (PCL) fillers. PPDO, PLLA, PCL, or PLGA/PLLA was injected into the dorsal skin of 8-week-old rats. Tissue samples were obtained 0, 2, and 4 weeks post treatment for Sirius Red-staining, polarized light microscopy, enzyme-linked immunosorbent assay (ELISA), real-time reverse transcription PCR (qRT-PCR), and western blotting. The MagBeads Total RNA Extraction Kit was used to isolate total cell RNA. The PLGA/PLLA filler demonstrated similar neocollagenesis and inflammatory response as other collagen stimulants, and showed better biodegradability than PPDO, PLLA and PCL fillers. Our data suggest that the newly developed collagen-stimulating PLGA/PLLA filler may be a safe and effective option for correcting volume loss and rejuvenating photoaging skin.

Radiolabeled somatostatin analogue is a useful ligand for scintigraphic imaging of somatostatin receptor-bearing tumors. In this study, we investigated the effects of different radiolabeling conditions on labeling yield and ratio between mono-iodinated and di-iodinated 125I Tyl 3-octreotide by HPLC analysis. In vitro and in vivo stabilities of 125I Tyr3-octreotide and 11 lin_DTPA_D_Phel_octreotid e were also determined. Both radiolabeled compounds were relatively stable in vitro, but were decomposed to free 125I and 11 lin_DTP A in vivo, respectively.

The behavior of 153Sm-EDTMP in vitro and vivo is analyzed by the size exclusion HPLC. The experimental results show that EDTMP amounts have an obvious effect on the stability in vitro and uptake of 153Sm-EDTMP in the liver. HPLC analysis of urine sample indicates that 153Sm-EDTMP is excreted in the original form. The behavior in vivo of 153Sm-EDTMP containing 4 ~tg is similar to that of 153Sm-EDTMP containing 50 ~tg EDTMP at 1 h post-injection.1

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