In this study, the staged seismic performance evaluations were conducted to the 91 high speed railway tunnels in use for checking whether to comply with the recent design criteria or not. In addition, the seismic fragility functions of the tunnels were developed to allow the probabilistic risk assessment. The results of the staged seismic performance evaluations which consist of a preliminary assessment and a detailed assessment, show that the tunnels comply with the recent design criteria. With reference to the results of previous studies, a form of the proposed seismic fragility functions was set as a log-normal distribution by PGA, and the parameters of the functions were determined by using the probability of damage for the design PGA level. The seismic fragility functions were developed for each types (Cut & Cover, NATM) of tunnels. The seismic fragility functions from this study and the existing research results (FEMA, 2004) were compared to evaluate the seismic performance level of the tunnels, as a result the tunnels of this study were relatively superior to the ASSM tunnels on the seismic performance.