This work reports a microfluidic device with deterministic lateral displacement (DLD) arrays allowing rapid and label-free cancer cell separation and enrichment from diluted peripheral whole blood, by exploiting the size-dependent hydrodynamic forces. Experiment data and theoretical simulation are presented to evaluate the isolation efficiency of various types of cancer cells in the microfluidic DLD structure. We also demonstrated the use of both circular and triangular post arrays for cancer cell separation in cell solution and blood samples. The device was able to achieve high cancer cell isolation efficiency and enrichment factor with our optimized design. Therefore, this platform with DLD structure shows great potential on fundamental and clinical studies of circulating tumor cells.

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We developed a spectroscopic monitoring project to investigate the kinematics of the broad-line region (BLR) in active galactic nuclei (AGN) with ultra-fast outflows (UFOs). Mrk 79 is a radio-quiet AGN with UFOs and warm absorbers, had been monitored by three reverberation mapping (RM) campaigns, but its BLR kinematics is not understood yet. In this paper, we report the results from a new RM-campaign of Mrk 79, which was undertaken by Lijiang 2.4-m telescope. Mrk 79 is seeming to come out the faint state, the mean flux approximates a magnitude fainter than historical record. We successfully measured the lags of the broad emission lines including Hβ λ4861, Hγ λ4340, He II λ4686 and He I λ5876 with respect to the varying AGN continuum. Based on the broad Hβ λ4861 line, we measured black hole (BH) mass of M • = 5.13 +1.57 −1.55 ×10 7 M ⊙ , estimated accretion rates ofṀ • = (0.05 ± 0.02) L Edd c −2 , indicating that Mrk 79 is a sub-Eddington accretor. We found that Mrk 79 deviates from the canonical Radius−Luminosity relationship. The marginal blueshift of the broad He II λ4686 line detected from rms spectrum indicates outflow of high-ionization gas. The velocity-resolved lag profiles of the broad Hγ λ4340, Hβ λ4861, and He I λ5876 lines show similar signatures that the largest lag occurs in the red wing of the lines then the lag decreases to both sides. These signatures should suggest that the BLR of Keplerian motion probably exists the outflow gas motion. All findings including UFOs, warm absorbers, and the kinematics of high-and low-ionization BLR, may provide an indirect evidence that the BLR of Mrk 79 probably originates from disk wind.