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Introduction According to the different melting point, palm oil can be divided into palm stearin 44-56 , intermediate component 26-43 and palmitin 24. After being fractionated by acetone and other organic solvents, palm stearin can be obtained with high-content tripalmitin PPP of triglyceride. Tripalmitin and oleic acid methyl oleate, methyl oleate can be used to synthesize 1,3-dioleoyl-2-palmitoylglycerol OPO structured lipid by enzymatic acidolysis reaction 1, 2. OPO could be used as human milk fat substitute HMFS. And that human milk was a natural emulsion system 3. Human milk fat HMF was the richest energy source for infants, supplying about 50-60 of dietary calories. Approximately 98 of the HMF was in the form of triacylglycerol TAG with a unique fatty acid tribution. Dietary TAGs are hydrolyzed into 2-monoacylglycerol 2-MAG and free fatty acids by pancreatic lipase in the intestine.