Background. With the improvement of people’s living standards, how to maintain health and delay aging to improve the quality of life and achieve longevity has become a hot topic of concern. objective. To investigate the effect of acupuncture combined with exercise on the expression of immune factors in aging rats and its significance in antiaging intervention. Materials and Methods. Forty-three SD rats included 12 rats in the control group, and the remaining rats were injected intraperitoneally with D-galactose 500 mg/kg to prepare a subacute aging rat model. The 24 rats that were successfully modeled were divided into acupuncture exercise groups and exercise groups according to the random number table method, with 12 rats in each group. After the modeling, the comparison group did not do any intervention, the exercise group was given aerobic exercise intervention, and the acupuncture exercise group was given acupuncture combined with exercise intervention. The effect of immune factor expression in rats was compared. Results. The levels of IgM, IgA, and IgG in the acupuncture exercise group were significantly higher than those in the exercise group ( P < 0.05 ). The IL-10 content in the acupuncture exercise group was significantly higher than that in the exercise group ( P < 0.05 ) and was significantly reduced in the acupuncture exercise group compared with the comparison group ( P < 0.05 ). The level of IL-6 in the acupuncture exercise group was significantly lower than that in the exercise group, and the level of IL-6 in the acupuncture exercise group was significantly increased compared with the comparison group ( P < 0.05 ). The C3 and C4 levels in the acupuncture exercise group were significantly higher than those in the exercise group ( P < 0.05 ). The levels of IFN-γ and TNP-α were significantly lower in the acupuncture exercise group than in the exercise group and significantly increased in the acupuncture exercise group compared with the comparison group ( P < 0.05 ). Conclusion. Ling turtle eight method acupuncture combined with exercise promoted the development of immune organ spleen, enhances the body’s immune function and complement system, inhibits the immune inflammatory response and regulates immune balance, reduces the inflammatory response caused by the aging of D-type galactose, and achieves the effect of delaying aging.