In this study, we investigated the effect of genotype  environment interaction (GEI) on the grain yield of 15 different sesame genotypes on four test areas (Arak, Birjand, Karaj and Shiraz) in two years (2017-2018). We observed significant differences in all sources of combined variance analysis. The interaction items in AMMI (additive main effects and multiplicative interactions) analysis of variance were divided into three parts, in which the interaction principal components IPC1 and IPC2 items justified about 85% of interactions. Also, IPC2 and residual items were non-significant. Our experiments showed that the superior genotypes were G8 > G13 > G9 > G10 > G15 > G14 > G7. The labile locations were Karaj > Birjand > Arak > Shiraz. Furthermore, G4, G1, G13, G8 and G10 (G4 > G8 > G9 > G10 > G13 > G6, respectively) had the least GEI concerning IPC1 vs. grain yield (IPC1 vs. IPC2, respectively). The recommended genotypes for each region include G9, G2, G3 and G12 for Arak and Birjand; G7, G11 and G14 for Karaj; and G10, G8, G4 and G13 for Shiraz. According to the low environmental impact and the proximity of the IPC values of Shiraz, Arak and Birjand environments, Arak can be considered as the mega-environment for these three locations. The stable genotypes with higher general durability consisted of G4 > G6 > G1 > G2. Based on the AMMI stability value (ASV) and genotype selection index (GSI), Jirouf 13 (G4), Darab 14 (G8), Safi Abad 1 (G9), Ahwaz Local Cultivar (G10) and Isfahan Local Cultivar (G13) were superior genotypes about grain yield and GEI impact, and can be recommended for future investigations.