I. INTRODUCTION Studies which have been reported from this laboratory on the relation of hydrogen ion concentration to viability of Bact. coli (Winslow and Falk, 1923) have suggested the great importance of certain amphoteric reactions of the bacterial cell. The experiments recorded here were undertaken with the view of studying, from anothqr angle, certain anomalous results of the studies
PROCEEDINGSEleven control subjects taking the same food at the same time without addition of filtrates remained well, but one reported the occurrence of food poisoning symptoms. Since this individual had been made quite ill with filtrates the previous week, the second attack may have been due to suggestion or to association with those who were ill.The toxic substance in staphylococcus filtrates that causes food poisoning is destroyed by boiling and either destroyed or greatly weakened by heating at 60-65°C. for 30 minutes.A second attack has been produced in one and the same individual by feeding a second portion of the same filtrate after i i week's interval.
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