Evaluation of ecosystem services has become a hotspot in terms of research focus, but uncertainties over appropriate methods remain. Evaluation can be based on the unit price of services (services value method) or the unit price of the area (area value method). The former takes meteorological factors into account, while the latter does not. This study uses Kunyu Mountain Nature Reserve as a study site at which to test the effects of climate on the ecosystem services. Measured data and remote sensing imagery processed in a geographic information system were combined to evaluate gas regulation and soil conservation, and the influence of meteorological factors on ecosystem services. Results were used to analyze the appropriateness of the area value method. Our results show that the value of ecosystem services is significantly affected by meteorological factors, especially precipitation. Use of the area value method (which ignores the impacts of meteorological factors) could considerably impede the accuracy of ecosystem services evaluation. Results were also compared with the valuation obtained using the modified equivalent value factor (MEVF) method, which is a modified area value method that considers changes in meteorological conditions. We found that MEVF still underestimates the value of ecosystem services, although it can reflect to some extent the annual variation in meteorological factors. Our findings contribute to increasing the accuracy of evaluation of ecosystem services.