We constructed a framework map of the edible mushroom Lentinula edodes (shiitake mushroom) using tetrad analysis. The map is based on the segregation of 264 randomly amplified polymorphic DNA (RAPD) markers, 14 structural genes, 1 expressed sequence tag (EST) marker, 2 mating factors (matA and matB), and 8 sequence-characterized amplified regions (SCARs) among 92 basidiosporic strains in 23 tetrads. We identified 11 linkage groups on a support interval of a minimal LOD score of 3.0 and a maximum distance of 25 centimorgans (cM). The length of the 11 linkage groups (LGs) ranged from 157.2 cM to 24.4 cM, and they covered a distance of 908.8 cM. One of the quantitative trait loci controlling the vegetative growth rate on potato dextrose agar (PDA) for L. edodes was detected between the markers D18CCA-360t and S08-1000c on LG2.