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of rpoE and rne induced by heat shock stress…………………………….. 3.2.4.3.3 Similar expression of promoter fusions in strains MG1655 and SCC1………………….. 3.2.4.4 Visualization of promoter activaties in biofilm cultures 3.2.4.5 Gfp expression of SCC1 carrying promoter-AsRed2 fusions……………………………… 3.3 Study of interspecies interactions with the established dual fluorescence system……………………………………………. 3.3.1 Promoter activities of E. coli in planktonic co-cultures………. 3.3.1.1 Influence conferred by K. pneumoniae seem to override that of E. faecalis…………………………….. 3.3.1.2 Promoter activity reduction cannot be totally attributed to species dominance………………………………….. 3.3.2 Promoter activities of E. coli in biofilm co-cultures………….. 3.4 Exploring the possible limitations of the dual fluorescence system… 3.4.1 Influence of various factors on green fluorescence of E. coli SCC1…………………………………………………... 3.4.2 Expanding the reporter capability of AsRed2…………………. 3.4.2.1 AsRed2 mutant DNA library…………………………... 3.4.2.2 Analyses of clones of interest from AsRed2 mutant library………………………………… Chapter 4 Discussions and future work………………………………. 4.1 The strengths of the system…………………………………………. 4.1.1 Suitability of E. coli SCC1 as reporter strain…………………. 4.1.2 Detection capacity when species of interest is in minority……. 4.1.3 Ability to reveal heterogeneity in gene expression……………. 4.1.4 Complementary data to the analysis of transcriptional response 4.1.5 Application of the system at the genomic level………………..