Flower  samples of Calendula officinalis were collected from the gardens of the college of Pharmacy in Baghdad city  to examine their antibacterial activity .The effect of ethanol crude extract of petals and reproductive parts of flowers in different concentrations were obtained and bioassayed in vitro for its bioactivity to inhibit the growth of eight types of bacteria .The extracts of petals  part were clearly superior for all bacteria especially the bacteria Pseudomonas aeruginosa (inhibition zone was 25mm in the concentration 100mg/ml) from gram negative bacteria, and Staphylococcus aureus (inhibition zone was 14mm in the concentration 50mg/ml) from gram positive bacteria, while the extracts of reproductive parts were less effective than petals part. These results confirm the antibacterial activity of Calendula officinalis flowers and support the traditional use of the plant in the therapy of bacterial infection

This study was include the investigation of the antibacterial activity of ethanolic extract for (12) type of Iraqi plant leaves (Allium porrum L, Apium graveolens Mill., Cassia angustifollia Vahl., Lawsonia inermis L., Lepidium sativum L., Malva sylvestris L., Monthapiperita L., Ocimmum bassillicum L., Pettroselinum crispum Mill., Raphanus sativus L., Sponacia oleraceal. and Trigonella foenum L.). In different concentrations (20, 40, 60, 80, 100) mg/ml against Salmonella typhimurium in vitro using agar well diffusion method. All ethanolic extract of leaves showed a high activity against this bacterium except of Cassia angustifollia Vahl. as compared with the other extracts. The ethanolic extract of leaves of Petroselinum crispum Mill was showed the best activity with inhibition zone of twenty three mm in the concentration (100) mg/ml. This results confirm the antibacterial activity of these plants, and leaf components which are responsible for this action have to be isolated for using as therapeutic agents.Conclusion: The present investigation together with previous studies provide support to the anti-Salmonella typhimurium properties of studied plant leaves. It can used as antibacterial supplement towards the development of the new chemotherapy . Additional in vivo studies and clinical trails would be needed to justify and further evaluate the potential of these plants as anti-Salmonella typhimurium agent in treatment of infectious diseases caused by this bacteria.

Microbial lipases today occupy a place of prominence among biocatalysts owing to their ability to catalyze awide variety of reactions in aqueous and non- aqueous media, A.baumannii were isolated from different clinical specimens from hospitalized patients from Baghdad hospitals and were detected by biochemical tests and API20E system. The percentage of isolation was (16.6%), A. baumannii is an increasingly multidrug – resistant (MDR), it showed high level of resistant to Ceftriaxon, Colistin, Piperacillin, Co-trimoxazol, Tertracycline, Carbenicillin, Amoxicillin, Penicillin G, Gentamicin and Ceftazidim , wherease the isolates were highly sensitive to Imipenem, Ciprofloxacin, Meropenem, Amikacin, and Cefotaxime. The isolated strians of A.baumannii were screened for the production of lipase by using Rhodamine B agar media. one of the isolated strians exhibited a greater clear zone than the others, indicated higher lipase activity.  The lipase in present study was partially purified by ammonium sulphate at the concenterations of 30% and 80%, the 30% ammonium sulphate showed higher activiy of the enzyme than 80% in which the enzyme activity was slightly decreased indicating that the better purification was at the 30% concenteration of ammonium sulphate.Various physicochemical parameters such as pH, temperature and metal ions were studied in order to determine the optimum conditions for lipase production. The production of lipase by A. baumannii was optimum at 35°C, pH 7.0 and was enhanced by the Ca++ and Na+ wherease inhibited by Zn++ ions. Key words : Acinetobacter baumannii, Lipase enzyme, Partial purification.

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A total of 10 samples involving five bread and five rice types and five cooked rice were selected. The iron and Zn contents were analyzed using an Inductively Coupled Plasma Mass Spectrophotometer (ICP-MS), and phytic acid content was analyzed using a rapid colorimetric method using a spectrophotometer. Phytic acid to Fe and Zn molar ratios were also determined using their molar weight to determine the potential bioavailability of selected nutrients. A one-way ANOVA test was used to statistically analyze the means differences between the phytate and mineral contents between the bread and rice samples. The variation of Fe and Zn (mg kg-1) in the bread samples was 24.1-65.6 and 13.3-22.8, in row rice was 10.8 to 45.3 and 8.66-17.4, and in cooked rice was 10.0-45.2 and 6.03-15.5 respectively. The PA concentration in (g 100g-1) was 0.77-1.14 for bread, 0.61-1.10 for row rice, and 0.31-0.77 for cooked rice. And also, PA/Fe and PA/Zn ratios were 2.58-6.01 and 8.07-13.4 for bread, 0.25-1.35, and 39.7-110 for row rice, and 0.11-0.89 and 23.0-125 for cooked rice, respectively. The results indicated that soaking and cooking processes caused the reduction in the Fe, Zn, and PA concentrations by 22.4, 5.54, and 27.7 %, respectively. These results from PA/Fe and PA/Zn molar ratios show that Fe had moderate bioavailability while Zn had low bioavailability. The results may be valuable for generate cultivars of wheat and rice varieties with suitable concentrations of PA and micronutrients, which can lead to the improvement of micronutrient-rich cultivars to reduce malnutrition.