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The temperature-sensitive cell division cycle (cdc) G1 mutants cdc28 and cdc35 show decreased mitochondrial volumes with respect to the wild type strain A364A (WT) at the restrictive temperature. Of the three criteria of mitochondrial biogenesis studied, that is, number of mitochondria per cell, relative area of the cell occupied by mitochondria, or relative area of mitochondria occupied by inner membranes, only the second indicator was significantly lower in cdc mutants than in the WT. The mitochondrial inner membranes development did not compensate for the decrease in the organelles volume. Apparently, the reduced mitochondrial biogenesis was not due to the temperature shift because the relative area of the cell occupied by mitochondria was already significantly lower at 25 degrees C in cdc mutants. The specific fluxes of oxygen consumption confirmed that the respiratory capacity of cdc mutants is largely impaired in respect to the WT. Cdc28 and cdc35 mutants of Saccharomyces cerevisiae had been previously shown to exhibit high respiratory quotients (from 3 to 7) in respect to the WT (RQ approximately 1.0), which correlated with carbon and energy uncoupling probably the result of glucose-induced catabolite repression [Aon MA, Mónaco ME, Cortassa S (1995) Exp Cell Res 217, 42-51; Mónaco ME, Valdecantos PA, Aon MA (1995) Exp Cell Res 217, 52-56].

The purpose of this report is to improve and make higher benefits on human diet through the consumption of seeds having high nutritional values. Different combinations of amaranth, chia and quinoa seeds and precooked soybean were used to elaborate a new sweet food. Mixed seeds (flour) and soybean whit peanut, sugar or stevia, glucose, hydrogenated oil and natural essence were prepared and tasted by people of both sex and age range from 1 to 78 years old. Previously nutritional composition was analyzed in the different samples. Sweet foods samples were given to persons to evaluate the acceptance and preference of them compared with two market candies of similar composition. The association analysis was performed using t-test and Analysis of Variance (ANOVA) with Bonferroni´s multiple comparisons for quantitative variables and chi square test for qualitative variables. From all the samples having a standard protein content (more than 10% each), have a higher acceptance those composed by amaranth, chia and quinoa, including more acceptance by women. The same result was obtained respect the preference. The production for human consumption of this new sweet food would imply a better use of vegetable proteins as a complement of the diet animal proteins and improve the health preventive advantages.

En Tucumán, Argentina, se desarrolló la producción de duraznos (Prunus persica) destinada al mercado local para consumo como fruta fresca y para la industria. La vida comercialde la fruta fresca es limitada y depende del tratamiento de conservación poscosecha. A fin de proponer un método para prolongar la vida útil de duraznos, se estudió el uso de coberturas deésteres de sacarosa, producto biodegradable de reconocido efecto en otras frutas. Lotes de duraznos seleccionados se trataron con soluciones acuosas de ésteres de sacarosa al 0,5% y 5%, conservándolos luego en frío. Se determinaron las siguientes variables de calidad: pérdida de peso, sólidos solubles totales y tamaño, comparando con duraznos sin tratar. Los resultados obtenidos con soluciones de ésteres de sacarosa al 5% evidenciaron: reducción en la pérdida de peso fisiológica y mayor diámetro en las frutas tratadas. Las variaciones de sólidos solubles son favorables en duraznos tratados. Soluciones de ésteres de sacarosa al 0,5% no ejercen efecto y causan alteraciones en las muestras. Se concluye que la refrigeración de duraznos frescos previamente tratados con soluciones de ésteres de sacarosa al 5% resultaría favorable para su conservación e incrementaría la duración y disponibilidad de los mismos en el mercado.

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