The structure of a trifunctional linker is shown. The linker contains a fluorescent dye (green), two palmitoyl moieties (light blue) and is covalently coupled to a protein (schematically shown in dark blue). The tripartite is used to study the effect palmitoylation on the localization of membrane proteins or peptides in giant‐unilamellar vesicles (GUVs) composed of a phase‐separating lipid mixture. Here, the palmitoylated protein is shown to localize to the liquid‐disordered part of the membrane (thinner membrane) as shown by the co‐localization with a specific lipid probe (red). More information can be found in the full paper by G. Roelfes, B. Poolman et al. on page 1320 in Issue 9, 2020 (DOI: 10.1002/cbic.201900655).

Scheme1.Trifunctional linker used to conjugate 1) DPPE via amide coupling, 2) Sulfo-Cy3 azide via CuAAC, and 3) WALP via maleimidecoupling.Scheme2.Synthetic route toward scaffold 5.

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