Immunity to autologous protein has not previously been described following nonhuman primate cardiac transplant. Native hearts and cardiac allografts from cynomolgus monkeys were assessed by immunohistology for vimentin, a highly conserved intermediate filament protein. IgM and IgG to vimentin were measured in serial sera from untreated (n = 4) or cyclosporine (CsA)-treated (n = 8, 2 with ATG) cardiac allograft recipients, and in groups treated with anti-CD154 antibody with (n = 6) or without ATG (n = 28). IgM or IgG reactive with vimentin was elaborated within 30 days with unmodified acute rejection (3/4) or in CsA-treated animals (5/6). CD154 blockade did not prevent anti-vimentin IgM (14/28) but tended to delay the IgG response during therapy (anti-CD154: 8/28, p = 0.10 vs. CsA; anti-CD154+ATG: 2/6). CAV and alloantibody were seen in 25 of 26 animals with grafts surviving over 30 days, including seven animals without increasing anti-vimentin antibody. Anti-vimentin antibodies and vascular complement deposition were found in rejected hearts. Acute and chronic alloimmunity disrupt modulation of autoreactivity to vimentin through pathways, which are resistant to CsA, but may be partially regulated by CD154.

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According to the traditional morphological classification, the Rhizoma Chuanxiong quality of traditional chinese medicine is divided into recommended products and non-recommended products; the chromatogram data of Rhizoma Chuanxiong are obtained in the standard condition, we inspect and abstract main data, get the main peaks of linear independent vectors. Get every clustering the most likely data of Rhizoma Chuanxiong with Particle Swarm Optimization of synchronous learning factor and Rosenbrock method of Optimization. Make Rhizoma Chuanxiong criterion data as grey-whitened Gumbel distribution and calculate similarity with the sample data of Gumbel distribution and ensure the finial similarity by weighted average. The program will use Simpson double integra and B-spline interpolation. It need to calculate the similarity between unknown sample and the common pattern of corresponding standard sample when analyze unknown sample. This research combines the plant morphology, chemistry, statistics and calculating technology establishing the pattern identification methods of quality, have good attempt to traditional chinese medicine quality assessment methods and make a basic to the deeper research about Rhizoma Chuanxiong in theory and practice.

According to the traditional morphological classification, the North Schisandra quality of traditional chinese medicine is divided into recommended products and non-recommended products; Discrete the chromatography data of the North Schisandra which obtained under the condition of standard test and also make the knowledge reduction. Obtaining the great peaks of linear independent vectors and obtaining every clustering centre data of North Schisandra by the fuzzy and Defuzzification methods. Make North Schisandra criterion and sample data as Normal-Weibull distribution to calculate similar. This research combines the plant morphology, chemistry, statistics and calculating technology to establish the pattern identification methods of quality and have good attempt to Chinese traditional machine quality methods. The research results provide the basis for medicine industry and manufacturing.