Based on The Mammalian Fauna of Guizhou, historical literature, and the latest research on taxonomy and phylogeny of bats, we provide an updated checklist on the diversity and distribution of bat species in Guizhou Province, China. A total of 51 bat species belonging to 7 families and 17 genera have been recorded in Guizhou as of 2015, which represents 38.1% of bat species (51 of 134 species) in China. Among these, 8 species are endemic to China, and 2 species only occur in Guizhou. When compared to bat species records in The Mammalian Fauna of Guizhou, we added 14 species, excluded 3 species, and renamed 10 species in this revised checklist. According to the animal geographical regions of China, 42 species belong to the Oriental Realm, 2 species are classified in the Palaearctic Realm, and the rest of species are considered widespread. Forty-four recorded species are listed in the endangered categories of the IUCN Red List, whereas 48 species are ranked in the Red List of China's Vertebrates.
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