AIM:To evaluate the hepatoprotective activity of a hydroalcoholic extract of the bark of Anogeissus latifolia ; in vitro in primary rat hepatocyte monolayer culture and in vivo in the liver of Wistar rats intoxicated by carbon tetrachloride (CCl4). METHODS:In the in vitro study, a primary hepatocyte monolayer culture was treated with CCl4 and extract of Anogeissus latifolia . Hepatoprotective activity was demonstrated in the CCl4 damaged primary monolayer culture. In the in vivo study, the hepatoprotective activity of a hydroalcoholic extract of Anogeissus latifolia was analyzed in liver injured CCl4-treated rats. Biochemical parameters including serum transaminases [aspartate aminotransferase (AST) and alanine aminotransferase (ALT)] and alkaline phosphatase (ALP) in serum were analyzed. The biochemical findings were supplemented with histopathological examination of rat liver sections.