Captive propagation and augmentation of the Rio Grande Silvery Minnow Hybognathus amarus is necessary for continued persistence of the species until habitat can be restored. Augmentation occurs using captive fish within the first year (age 0) through age 2; however, older year‐classes (ages 3–4) have been released into the wild. We quantified fecundity and compared egg quality across four reproductive age‐classes (1–4) of hatchery‐reared Rio Grande Silvery Minnow. Batch fecundity (total number of spawned eggs) ranged from 2,029 eggs in age‐1 fish to 10,588 eggs in age‐4 fish. Standing stock of vitellogenic (i.e., yolked) oocytes remaining in ovaries after spawning increased from 988 oocytes at age 1 to 4,924 oocytes at age 4. Total fecundity (i.e., batch fecundity plus standing stock of yolked oocytes) increased from 3,017 eggs and yolked oocytes in age‐1 fish to 15,522 eggs and yolked oocytes in age‐4 fish. Of note, batch fecundity ranged from 57% to 68% across the four age‐classes indicating that over half of the total fecundity occurs in the first spawn. Average percent fertilization of eggs (63–82%) was not detectably different across the four age‐classes, while average egg diameter (4.1–5.5 mm) increased as age increased. Fecundity increased across all four reproductive age‐classes of Rio Grande Silvery Minnow, with the larger portion of total fecundity occurring in the first spawn across all age‐classes. The timing of spawning with optimal conditions in the Rio Grande is critical for survival and ultimately recruitment into the wild fish population.