ÖZET Amaç: Bu çalışmanın amacı basit bir besiyeri olan Mueller-Hinton agar (MHA)'ın germ tüp testinde (GTT ABSTRACT Aims: The aim of this study is to evaluate Mueller-Hinton Agar (MHA) as a simple medium for the determination of germ tube test (GTT). Methods: A total of 86 Candida species isolated from different clinical specimens were included. All isolates were cultured onto Sabouraud Dextrose Agar (Salubris, Turkey) and incubated at 37°C for 24-48 hours. They were identified by VITEK MS (Biomérieux, France Candida albicans, one C. dubliniensis, 12 C. parapsilosis, 12 C. glabrata, 4 C. kefyr, 3 C. tropicalis, 3 C. krusei. Serum GTT was positive in 30 (58.8%) and 36 (70.6%)