Objective: The present study aimed to assess of four liver enzymes, Alanine Aminotransferase (ALT), Aspartate Transaminase (AST), Alkaline Phosphatase (ALP) and Gamma-Glutamyl Transpeptidase (GGT). Material and Methods: Based on periodontal clinical parameters, sixty four patients with chronic periodontitis (CP) and twenty four controls were enrolled in the study. Saliva and serum samples were collected and Automated Chemistry Analyzer AU 480 was employed to assess levels of enzymes. Results: Compared to healthy controls, the levels of the four enzymes were significant increased in serum of patients, especially in the severe group while in the saliva a significant increase observed only in the level of AST. Moreover, Alanine Aminotransferase (ALT), Aspartate Transaminase (AST), Alkaline Phosphatase (ALP) and Gamma-Glutamyl Transpeptidase (GGT) the levels of these enzymes in serum were significantly higher than those in saliva. Conclusion: ALT, AST, ALP and GGT serum levels are suggested to be important indicators for disease progression as well as predict the liver health.