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ANNALS NEW YORK ACADEMY OF SCIENCES 236 FIGURE 1. Nucleotide sequence of the ORF of the D-hydantoinase gene (dht) from Pseudomonas putida 7711-2. CHEN & TSAI: D-HYDANTOINASE 237 FIGURE 2. Deletion analysis for localization of the dht gene on a 3.3-kb SalI-PstI DNA fragment. Insert DNA (open boxes), ORF of dht gene (➨), and deleted segments (½½) are indicated. The D-hydantoinase activity of various derivatives is qualitatively shown on the right. Numerals indicate nucleotide sequence number.