We investigated the roles and biochemical properties of recombinant murine norovirus-1 (MNV-1) 3D pol in RNA synthesis and virus genome-linked protein (VPg) nucleotidylylation. We therefore was the probable target site of guanylylation. Homopolymeric RNAs did not enhance VPg guanylylation, whereas in vitro-transcribed (") subgenomic (SG) and (+)SG RNA enhanced VPg guanylylation by 9.2 and 3.2 times, respectively. Within (")SG RNA, the (")ORF3 region played a critical role in enhancing VPg guanylylation, suggesting that the MNV-1 ORF3 region of negativestrand RNA contains a cis-acting element that stimulates 3D pol -mediated VPg guanylylation.

No abstract

A Korean nationwide surveillance on circulating rotavirus strains was conducted from September 2000 to August 2007 aiming to obtain prevaccine data for predicting vaccine effectiveness. The predominant strains among the 2779 strains analyzed varied annually and only approximately 50% had either a G or a P antigen present in both RotaTeq (Merck & Co. Inc., Whitehouse Station, NJ, USA) and Rotarix (GlaxoSmithKline, Brentford, UK).